Methods and Applications in Fluorescence

荧光的方法和应用是荧光光谱、成像、荧光探针、标签和材料领域的原始研究文章、综述文章和技术说明的论坛。它的重点是方法和（高级）应用。以下主题是其报道的代表。光谱学：生物分子的发光光谱，天然和合成荧光团，荧光寿命和偏振；共振能量转移（FRET）；相关光谱；猝灭；上转换；等离子体相互作用；多光子激发；单分子光谱，激光诱导荧光光谱，高级组件和仪器、纳米测量等。成像：荧光显微镜；多光子显微镜、定量荧光显微镜（如FLIM、FCS、FRAP、TIRF）；单分子显微镜；超分辨率显微镜（如STED、PALM、STORM、GSD、SIM）、3D成像；单平面照明显微镜、体内荧光成像；先进的成像设备和系统等。标签、探针和传感器：蛋白质和DNA标签；插层器；神经递质和激素标签；离子探针；发光螯合物和金属有机探针；生物结合；镧系荧光、荧光蛋白质；膜和脂质探针；（分子）化学传感器和生物传感器、酶探针；（生物）分析（诊断，排序，信号，筛选）等。材料：包括各种点的纳米材料；发光纳米颗粒；纳米管；团簇；发光聚合物、镧系发光材料等。该杂志不会考虑概述荧光的常规用途（例如，在检测中）、纯描述性工作或合成的文章，而不演示其用途。

Methods and Applications in Fluorescence is the forum for original research articles, review articles and technical notes in the area of fluorescence spectroscopy, imaging, fluorescent probes, labels and materials. Its focus is on both methods and (advanced) applications. The following topics are representative of its coverage.Spectroscopy: Luminescence spectroscopy of biomolecules, of natural and synthetic fluorophores, fluorescence lifetime and polarization; resonance energy transfer (FRET); correlation spectroscopy; quenching; upconversion; plasmonic interactions; multiphoton excitation; single-molecule spectroscopy, laser-induced fluorescence spectroscopy, advanced components and instrumentation, nanometry, etc.Imaging: Fluorescence microscopy; multi-photon microscopy, quantitative fluorescence microscopy (such as FLIM, FCS, FRAP, TIRF); single-molecule microscopy; super resolution microscopy (such as STED, PALM, STORM, GSD, SIM), 3D imaging; single plane illumination microscopy, in vivo fluorescence imaging; advanced devices and systems for imaging, etc.Labels, probes and sensors: Protein and DNA labels; intercalators; labels for neurotransmitters and hormones; probes for ions; luminescent chelates and metal—organic probes; bioconjugation; lanthanide fluorescence, fluorescent proteins; membrane and lipid probes; (molecular) chemical sensors and biosensors, enzyme probes; (bio)assays (diagnostic, sequencing, signalling, screening), etc.Materials: Nanomaterials including all kinds of dots; luminescent nanoparticles; nanotubes; clusters; luminescent polymers, lanthanide luminescence, etc.The journal will not consider articles outlining routine uses of fluorescence, (for example, in detection), purely descriptive work, or synthesis without a demonstration of its use.