NATURE METHODS

《自然方法》是一个出版新方法的论坛，并对生命科学中久经考验的基础研究技术进行重大改进。这本月刊面向广泛的、跨学科的、积极参与实验室实践的学术和行业研究人员。它为他们提供了进行研究的新工具，并特别强调所提出的工作的直接实际意义。该杂志出版主要的研究论文，以及对最近技术和方法发展的综述。我们正在积极寻找与生物和生物医学科学相关的主要方法论文，包括基于化学的方法，对生物问题的研究有实际应用。为了提高每篇论文的实际相关性，必须在描述该方法的同时对其进行验证，将其应用于一个重要的生物学问题，并将结果与现有方法进行比较，说明其性能。选择发表的文章应具有广泛的兴趣，对方法的执行情况进行全面的评估，并提供全面的技术说明，以便立即加以应用。感兴趣的具体领域包括但不限于:核酸操作、扩增和测序方法蛋白质工程、表达和纯化方法蛋白质组学的方法，包括质谱分析、结合相互作用分析、基于微阵列的技术、显示技术、翻译后修饰分析、糖生物学和代谢组学系统生物学的方法，包括蛋白质组学方法、蛋白质相互作用分析方法和基因组广泛表达和调控谱生物分子结构分析技术，包括核磁共振和晶体学化学生物学技术，包括化学标记，扩展遗传密码和定向进化的方法生物物理方法，包括单分子和芯片上的实验室技术光学和非光学成像技术，包括探针设计和标记方法，显微镜，光谱学和体内成像基因表达的分析和操作技术，包括表观遗传学、基因靶向、转导、RNA干扰和基于微阵列的技术细胞培养和操作的方法，包括干细胞、单细胞方法和芯片上的实验室技术免疫学技术，包括产生抗体，基于抗体的检测和免疫标记研究包括癌症在内的生理和疾病过程的方法方法包括模型生物及其操作和表型用于分析、建模或可视化生物数据的计算方法和生物信息学方法基于纳米技术的方法应用于基础生物学

Nature Methods is a forum for the publication of novel methods and significant improvements to tried-and-tested basic research techniques in the life sciences. This monthly publication is aimed at a broad, interdisciplinary audience of academic and industry researchers actively involved in laboratory practice. It provides them with new tools to conduct their research and places a strong emphasis on the immediate practical relevance of the work presented.The journal publishes primary research papers as well as overviews of recent technical and methodological developments. We are actively seeking primary methods papers of relevance to the biological and biomedical sciences, including methods grounded in chemistry that have a practical application to the study of biological problems.To enhance the practical relevance of each paper, description of the method must be accompanied by its validation, its application to an important biological question and results illustrating its performance in comparison to available approaches. Articles are selected for publication that present broad interest, thorough assessments of methodological performance and comprehensive technical descriptions that facilitate immediate application.Specific areas of interest include, but are not limited to:Methods for nucleic acid manipulation, amplification and sequencingMethods for protein engineering, expression and purificationMethods for proteomics, including mass spectrometry, analysis of binding interactions, microarray-based technologies, display techniques, analysis of post-translational modifications, glycobiology and metabolomicsMethods for systems biology, including proteomics approaches, protein interaction analysis methods and genome wide expression and regulation profilingBiomolecular structural analysis technologies, including NMR and crystallographyChemical biology techniques, including chemical labeling, methods for expanding the genetic code and directed evolutionBiophysical methods, including single molecule and lab-on-a-chip technologiesOptical and non-optical imaging technologies, including probe design and labeling methods, microscopy, spectroscopy and in vivo imagingTechniques for the analysis and manipulation of gene expression, including epigenetics, gene targeting, transduction, RNA interference and microarray-based technologiesMethods for cell culture and manipulation, including stem cells, single cell methods and lab-on-a-chip technologiesImmunological techniques, including production of antibodies, antibody-based assays and immunolabelingMethods for the study of physiology and disease processes including cancerMethods involving model organisms and their manipulation and phenotypingComputational and bioinformatic methods for analysis, modeling or visualization of biological dataNanotechnology-based methods applied to basic biology