METHODS

每一期专题由一位在该领域具有专家资格的客座编辑组织，仅由专业作者邀请的高质量文章组成，其中许多是评论文章。这些问题专门讨论具体的技术方法，并强调对议定书的明确详细说明，以便容易地加以复制。所提供的背景资料使研究人员能够了解这些方法的基本原理;其他有用的部分包括提供特定方法的优点和缺点的替代方法的比较、避免潜在陷阱的指导和故障排除建议。方法着重于快速发展的实验生物学和医学技术。最近的一些专题讨论涉及这些热点问题:?染色质结构?使用TALE和CRISPR编辑和研究基因组?细胞定位和测量的工具和方法?利用组学数据的生物信息学的最新发展?核酸纳米技术?带有组学数据的系统生物学?自噬?神经科学中多模态数据的空间映射?推进分子细胞病理学的边界?NGS在翻译研究中的应用?使用质谱法检测和定量临床样本中的蛋白质

Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.Methods focuses on rapidly developing techniques in the experimental biological and medical sciences.Some recent thematic issues covered these hot topics:? Chromatin Structure? Editing and investigating genomes with TALE and CRISPR? Tools and Methods for Cellular Localization and Measurements? Recent development in bioinformatics for utilizing omics data? Nucleic Acids Nanotechnology? Systems Biology with Omics Data? Autophagy? Spatial mapping of multi-modal data in neuroscience? Advancing the boundaries of molecular cellular pathology? NGS applications to translational research? Detection and Quantification of Proteins in Clinical Samples Using Mass Spectrometry